There is also a special kind of orthogonal projection, called skewed isometry. Basically it is a an isometric projection scaled 2x and rotated at 45 angle so that the diamonds become squares, which allows tiling them easily with the simpler RPGMaker like engine, without actual isometric engine, while at the same time directional movement is more intuitive: up is still up on d-pad, and north is north. It is a bit easier to draw some art assets in this projection, which gives the distinct artsy look, while maintaining the proper isometric engine 3d capabilities (Z-axis can be visualized easily). It is nauseating to look at for RPG games, but could work with a bio-themed game.

It can be produced in several ways. Another is rotating the proper isometric render by -15 degree, then skewing/sheering 2x (Nth scanline moved 2N pixels to the left).