Yes you can do it...

In Graphics Gale there are two areas that SEEM similar but are different...
I work with graphics gale almost every day...

The two areas are: Layers and Frames

If you want to create a Transparent Gackground in the final saved product, which is what I assume you are asking, then what you need to do is:

Click the Properties for any Frame.
Click the checkbox for:  All Frames
Goto the next section called: Transparent
Click the checkbox for:  Enabled
Then select the color that you want to be Transparent

If you save as a GIF or PNG every Frame that has the color you selected in it will turn that color Transparent...

Hope that helps...