In the run animation, the eye seems to bounce half a pixel up. Are you not drawing these at 1x?
The AA colours aren't really helping because they're too light. Try making them a tad darker, especially on the green one.

The pink looks good but the greeb AA us stukk barely visible.

The run bobbing feels off to me, it's too quick and I'm not quite sure that it happens at the right point in the cycle.

Darker.

Remember, AA is essentially blending between two colours. It should be be approximately halfway between them, value-wise. It doesn't have to be exactly halfway since hues and surrounding colours also play into it, but it's a good starting point. Your "darker" AA was almost as bright as the light green.
I hue-shifted the colour towards blue and desaturated it a bit just because that looks better.

The new animations look a lot better!

I recommend not upscaling your art like you have been, just upload it at 1x. Your resizing is making some of the pixels wonky.

Possible transformation animation

Couldn't access this site for some reason, but yeah that is something I plan on doing actually! I kinda want them to have power-ups/transformations that can help eachother out with attacking and mobility.



This would be when the pink one jumps on him, not too sure if the bounce is portrayed well though.

Also that's a cute animation there

Ahh thank you!

Still getting things together but here's a basic default attack.


Also putting this specific game on hold for another, so expect different sprites at some point!