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Way to go! =)
Next, consider keeping banding in check. In this piece, most of it arises from too extensive AA: the pixel clusters align in a disturbing way. Making most of them shorter by 1 px won't hurt.
Also, some of the linework is still a bit jaggy, esp the upper right side of the face.
Have reduced the colours to 16 and reworked the colouring.
Thank you so much for the feedback, I really appreciate it! 
Will work on it some more to improve.
Cute and nicely pixelled, welcome!
32 colors is a lot however, many of them are very similar. Could be done with 16 ;)
Despite this the palette is missing a transition between the bright yellow and the nearest grey, it would help greatly with the anti-aliasing.
nice :)
tail anim looks good