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I like your colors, especially on the green and dark blue trees. Overall pretty solid.
If you want to improve it further, I think your base tree could be a ilttle more uneven in shape and volume, right now your trunk is basically a symmetrical X shape. I'd suggest picking what type of tree you want to make and looking for reference on that. Here's a trunk picture and you can see how the tree kind of leans a bit to one side more than the other and starts to splay out roots above ground, whether due to soil erosion or just how the tree has grown.
http://fc04.deviantart.net/fs70/i/2012/261/b/6/old_tree_trunk__stock_by_zummerfish-d5f40vm.jpg
Also your tree limbs are all pointing up. Many/most trees have limbs that point out to the side or even down, but again this is extremely dependant on what type of tree it is. A willow droops, a pine may be very straight sideways, an oak may be fat and sassy..I mean lumpy.
A picture of limbs in all directions
http://lib.colostate.edu/archives/agriculture/rmfu/photos/RMFU0070.jpg
As for an autumn tree that's losing leaves, how do remaining leaves clump on the tree? This image I found looks like the leaves at the end of branches on average are lasting longer. It's not a completely even distribution of remaining leaves.
http://daffodilphotoblog.files.wordpress.com/2011/11/leaves20111121_14zb.jpg
I think overall the more you can vary your design and look at reference the stronger these sorts of things will become. Variety is interesting in art, in pixel art it's even harder as you don't have a lot of space to show something while trying to create something interesting, but we all manage. :)
and I have to make everything alone. I was making those trees ~5 hours and I used:
It is such important knowlege, I will remember it and I will save those images
I just didn't think about "what type of tree is it", I just drew