I found the issue! It should be 25-29 colors. I also went back and tried to encorperate the eye colors the rest of the pallete. That's a neat tool you have there. Is there a way to use it before a piece is submitted?
I used a palette and placed each pixel. It has to be in the way I'm saving these animations or something. It shouldn't have as many as 191, but the eye is 4 pixels of a color not used anywhere else. :(
191 colors, many of them appearing in one pixel of one frame. 
Good linework and anim though.
I sent you a private message about it :D