Do you mean pixelart animation in Flash?
You can but you need to make the frames in PS as whole symbols. You can however take an item that moves and sometimes motion tween it if it moves in whole coordinates.
NOTE: that all PA needs a 1px transparent clearance and all lossy turned off when importing into the stage.

In Photoshop, pretty much the same thing using the internal ImageReady program.

For pixelart the main thing is that as long as items don't distort in angle (causing blurring) you can use the element. Every distortion needs to be a new item.

Hope that helps :D

Flash is designed to make very small files and always smooths symbols as much as possible (the reason for the smoothness when scaling). Whenever you use pixelart in a Flash movie you need to trick it into keeping your pixels sharp. In my experience the best way to do this is the following;

• Always 'save for web' in Photoshop. (to control the file size)
• Allow a 1px clearance of transparency around your pixel piece. For example, a pixelart that is 64x64pixels would be 65x65px. This allows Flash to do its thing on the edges (1px) and it leaves the pixels clean and sharp.
• Once imported and made a symbol, double-click the 'picture icon', not the symbol and turn off all auto-AA and lossy compressions.
• The symbol itself needs to always be at 0X,0Y coordinates for sharpness. (not centered!!!
• When using pixelart symbols on the stage the symbols are always set in whole number coordinates -1 from where you want it. Example, if your symbol is going on the top left corner 0X,0Y, then your symbol placement is -1X,-1Y.

one pixel at a time is the only way I'm afraid.