This is a cute scene! You have a single pixel above the fish on one frame that pops in and out (it's a black pixel, you can see it better on here: https://www.linneart.com/pj/?input=https%3A%2F%2Fpixeljoint.com%2Fpixelart%2F157514.htm )
Keep up the good work, tho! You have a good start here~
I think this piece is bigger that it needs to be since you are not doing much with the ammount of space you have more tan a few sticks, try to always use as much as you can from your canvas, the animation works fine but i think it might be a little stiff, great work tho i hope you continue improoving!